Chromatographic analysis species color In the spectral method, the mobile phase can be either a gas or a liquid, which can be divided into Gas chromatography (GC) and Liquid chromatography (LC). The stationary phase can be either solid It can also be a liquid coated on a solid, whereby gas chromatography and liquid chromatography can be further divided into gas-liquid chromatography, gas-solid chromatography, liquid-solid chromatography, and liquid-liquid chromatography. In addition, there is a supercritical fluid chromatography (supercritical fluid chromatography) which uses a supercritical fluid as a flow-through phase, a solid adsorbent (such as silica gel) or a high polymer bonded to a carrier (or capillary wall). The product is a stationary phase chromatography. Supercritical fluid is a state of matter above the critical pressure and critical temperature. It is neither a gas nor a liquid, but it has some properties of gas and liquid (Editor-in-Chief of Drug Chromatography, Dingli People's Health Publishing House, 2008) The first edition in July). High performance liquid chromatography The method (HPLC) is a new chromatographic technique that uses a liquid as a mobile phase after gas chromatography and developed in the early 1970s. High performance liquid chromatography is developed on the basis of gas chromatography and classical chromatography. There is no essential difference between modern liquid chromatography and classical liquid chromatography. The difference is that modern liquid chromatography is higher than classical liquid chromatography. effectiveness And automated operations. In classical liquid chromatography, the mobile phase is transported under normal pressure, and the stationary phase used has a low column efficiency and a long analysis period. Modern liquid chromatography cites the theory of gas chromatography, and the mobile phase is changed to high pressure Conveying (maximum delivery pressure up to 4.9 Ì107Pa); Column Is a special method with small particle size filler Filled to make the column efficiency much higher than the classical liquid chromatography (the number of plates per meter can reach tens of thousands or hundreds of thousands); at the same time, the column is connected with high sensitivity. Detector The effluent can be continuously tested. Therefore, high performance liquid chromatography has the characteristics of fast analysis speed, high separation efficiency and automation. So people call it high pressure, high speed, high efficiency or modern liquid chromatography. The HPLC system generally consists of a high performance liquid chromatograph consisting mainly of a sample introduction system, an infusion system, a separation system, a detection system, and a data processing system. Among them, high-pressure infusion pumps, columns, and detectors are key components. Some instruments include gradient elution devices, online degassers, autosamplers, pre-columns or guard columns, column temperature controllers, etc. Modern HPLC instruments and microcomputer control systems for automated instrument control and data processing. The preparative HPLC instrument is also equipped with an automatic fraction collection device. In honor of the 70th anniversary of the victory of the War of Resistance Jiedao Technology The sample bottle free gift will be launched on September 3rd. Please contact us for details. king! ! ! ! !
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