Hengyuan tells you how to reduce the error probability of ELISA kits - Database & Sql Blog Articles

How does the experimental error probability of the elisa kit shrink? In the process of operation, in addition to the need for more careful, there are some places that need to be paid attention to. Shanghai Hengyuan Bio has summed up nine aspects for you: â–³ Aspect 1: The inspection technician should have laboratory operation Basic qualities and practical experience. Proficiency in the operation of experimental instruments and instruments, with the ability to summarize and analyze problems, and to solve the accidents in the experiment in a timely manner. â–³ Aspect 2 Use a proofread micropipette to eliminate natural errors. Whether the pipette is accurate or not is especially important for quantitative detection. â–³ Aspect 3: Read the instructions carefully and carry out the standardization operation in strict accordance with the requirements of the manual. ELISA kits with different batches of reagents cannot be mixed. Where it is worth improving, trial and error can be improved after it is established. â–³ Aspect 4: Washing thoroughly wash plate is not complete, the enzyme conjugate background color will show false positive. The washing liquid should be fresh, and the existing washing liquid will have a background increase, and false positives may also occur. â–³ Aspect 5: Strictly controlled reaction time The reaction time is too long, the enzyme is inactivated; the reaction time is too short, the enzyme conjugate cannot be fully combined with the microbial antigen antibody in the serum, and the structure of the product is loose and not easy to wash off, which may cause False negative. â–³ Aspect 6 Note the shelf life of the ELISA kit. The reagents that have passed the shelf life cannot be used. â–³ Aspect 7: Evaluating the practicability of the reagent The stability of the reagent is important to the accuracy of the reagent. Before the reagent is activated, the yin and yang control and the sample repeated comparison test shall be carried out, and the reagent may be used after determining that the reagent meets the requirements. â–³ Aspect 8: Strictly control the color development time is too short, after the termination of the reaction, the amount of substrate conjugate is too small, prone to false negative. Color development after the time required for color development should be judged as a false positive, which may be related to the reagent itself. Immediately after the coloring agent is added, the color is developed, and no positive is reported. This may be the result of background color development. â–³ Aspect 9: The sample should be accurate and fast. If the sample is not accurate, the amount of the enzyme can not be determined, and the ELISA kit directly affects the color development result. The color depth and the determination of the A value are related to the amount of the developer and the stop solution added, so the sample should be used with caution. If the sample is loaded within a certain period of time, if the sample is sluggish, the error will occur and the reagent will be exposed for a long time. Especially when the room temperature is too high, the shelf life will be shortened or even invalid.